Volume: 54 Issue: 4
Year: 2023, Page: 68-72, Doi: https://doi.org/10.61649/kujos/v54i4.23.1
Received: Sept. 6, 2023 Accepted: Nov. 15, 2023 Published: Dec. 26, 2023
The current study aimed to evaluate the anti-obesity potential of Simarouba glauca leaf extract by using a 3T3-l1 cell line. Various assays were performed to check anti-obesity such as lipase inhibition assay, amylase inhibition assay, oil-red-o staining, and MTT assay to check the anti-obesity potential of leaves extract. The leaves were shade-dried and crushed into powdered form. Three different extracts such as ethanol, methanol, and hydroalcoholic extract were prepared by using the Soxhlet extraction method. The pure form of the extract was formed after Rota evaporation. Later anti-obesity activity was determined by using different assays. The ethanol extract of leaves showed higher anti-obesity potential on 3T3-l1 cell lines for lipase inhibition assay, amylase inhibition assays, oil red o staining, and cytotoxicity assay. Simarouba glauca is traditionally used for the prevention and treatment of many diseases such as cancer, dysentery, fever, and malaria.
Keywords: Simarouba glauca, Obesity, Lipase Inhibition Assay, Amylase Inhibition Assay, Oil-Red-O Staining, MTT Assay
Wang Y, Chen HJ, Shaikh S, Mathur P. Is obesity becoming a public health problem in India? Examine the shift from under‐ to overnutrition problems over time. Obesity Reviews. 2009;10(4):456–474. Available from: https://doi.org/10.1111/j.1467-789X.2009.00568.x
Keramat SA, Alam K, Sathi NJ, Gow J, Biddle SJH, Al-Hanawi MK. Self-reported disability and its association with obesity and physical activity in Australian adults: Results from a longitudinal study. SSM - Population Health. 2021;14:100765. Available from: https://doi.org/10.1016/j.ssmph.2021.100765
Atlantis E, Lange K, Wittert GA. Chronic disease trends due to excess body weight in Australia. Obesity Reviews. 2009;10(5):543–553. Available from: https://doi.org/10.1111/j.1467-789X.2009.00590.x
Bjerregaard LG, Adelborg K, Baker JL. Change in body mass index from childhood onwards and risk of adult cardiovascular disease,. Trends in Cardiovascular Medicine. 2020;30(1):39–45. Available from: https://doi.org/10.1016/j.tcm.2019.01.011
Blagojevic M, Jinks C, Jeffery A, Jordan KP. Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis. Osteoarthritis and Cartilage. 2010;18(1):24–33. Available from: https://doi.org/10.1016/j.joca.2009.08.010
Jensen W. The Origin of the Soxhlet Extractor. Journal of Chemical Education. 2007;84(12):1913. Available from: https://pubs.acs.org/doi/10.1021/ed084p1913
Avelar-Freitas BA, Almeida VG, Pinto MCX, Mourão FAG, Massensini AR, Martins-Filho OA, et al. Trypan blue exclusion assay by flow cytometry. 2014. Available from: https://doi.org/10.1590/1414-431X20143437
Zheng CDD, Duan YQQ, Gao JMM, Ruan ZGG. Screening for Anti-lipase Properties of 37 Traditional Chinese Medicinal Herbs. Journal of the Chinese Medical Association. 2010;73(6):319–324. Available from: https://doi.org/10.1016/S1726-4901(10)70068-X
Metre TV, Kodasi B, Bayannavar PK, Bheemayya L, Nadoni VB, Hoolageri SR, et al. Coumarin-4-yl‐1,2,3‐triazol‐4-yl-methyl-thiazolidine-2,4-diones: Synthesis, glucose uptake activity and cytotoxic evaluation. Bioorganic Chemistry. 2023;130:106235. Available from: https://eurekamag.com/research/080/657/080657227.php
Kim JS, Jeon WJ, You HJ, Park MS, Ji GE. Inhibitory activities of Rubi Fructus on digestive enzymes. Food Science and Biotechnology. 2010;19(5):1165–1170. Available from: https://doi.org/10.1007/s10068-010-0166-z
Kumbar VM, Muddapur UM, Bhat KG, Shwetha HR, Kugaji MS, Peram MR, et al. Cancer stem cell traits in tumor spheres derived from primary laryngeal carcinoma cell lines. Contemporary Clinical Dentistry. 2021;12:247. Available from: https://doi.org/10.4103/ccd.ccd_252_20
Chedda U, Kaikini A, Bagle S, Seervi M, Sathaye S. In vitro pancreatic lipase inhibition potential of commonly used Indian spices. IOSR Journal of Pharmacy. 2016;6:10–13. Available from: https://www.iosrphr.org/papers/v6i10V1/B0610011013.pdf
Sahib MN, Darwis, Peh, Abdulameer SA, Tan. Rehydrated sterically stabilized phospholipid nanomicelles of budesonide for nebulization: physicochemical characterization and in vitro, in vivo evaluations. International Journal of Nanomedicine. 2011;p. 2351. Available from: https://doi.org/10.2147/IJN.S25363
Chheda ZS, Sharma RK, Jala VR, Luster AD, Haribabu B. Chemoattractant Receptors BLT1 and CXCR3 Regulate Antitumor Immunity by Facilitating CD8+ T Cell Migration into Tumors. The Journal of Immunology. 2016;197(5):2016–2026. Available from: https://doi.org/10.4049/jimmunol.1502376
Roh C, Jung U. Screening of Crude Plant Extracts with Anti-Obesity Activity. International Journal of Molecular Sciences. 2012;13(2):1710–1719. Available from: https://doi.org/10.3390/ijms13021710
Bhutkar MA, Bhise S. In Vitro Studies on Alpha Amylase Inhibitory Activity of Some Indigenous Plants. Modern applications in Pharmacy & Pharmacology. 2012;1(4):457–462. Available from: https://www.tsijournals.com/abstract/in-vitro-assay-of-alpha-amylase-inhibitory-activity-of-some-indigenous-plants-11105.html
Puranik SI, Ghagane SC, Nerli RB, Jalalpure SS, Hiremath MB. Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line. Pharmacognosy Journal. 2017;9(6):906–912. Available from: https://doi.org/10.5530/pj.2017.6.142
Jeon G, Choi Y, Lee SMM, Kim Y, Jeong HSS, Lee JM. Anti-obesity activity of methanol extract from hot pepper (Capsicum annuum L.) seeds in 3T3-L1 adipocyte. Food Science and Biotechnology. 2010;19(4):1123–1127. Available from: https://doi.org/10.1007/s10068-010-0160-5
Sweta Gupta, Sridevi I Puranik, Rubeen Nadaf, M A Mujeeb, Bhushan Kulkarni, Abhijit Bhatkal, Ravindranath Aladkatti, Vijay M Kumbar, Shridhar C Ghagane. Investigation of Anti-obesity Potential of Simarouba glauca Leaves Extract Using in-vitro Method. Karnatak University Journal of Science 54(4), (2023), 68–72. https://doi.org/10.61649/kujos/v54i4.23.1