Volume: 54 Issue: 3
Year: 2023, Page: 58-63, Doi: https://doi.org/10.61649/kujos/v54i3.benarzee
Received: Oct. 29, 2022 Accepted: Nov. 23, 2022 Published: Nov. 15, 2023
Green synthesis of nanoparticles is rapidly evolving because it is cost effective and eco-friendly, this also contributes to its preference over nanoparticles from other sources. In this study, silver nanoparticles were synthesized from aqueous extracts of neem flower (NF) and the biological activities evaluated. The silver nanoparticles (AgNPs@NF) were synthesized from extracts respectively using silver nitrate and characterized using UV–visible spectroscopy (UV–Vis), Fourier transform infrared (FT-IR) spectroscopy, Powder X-ray diffraction (PXRD), scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDX). The FT-IR spectra of AgNPs@NF showed characteristic groups pertaining to active molecules of extract indicating their surface functionalization. The XRD pattern of AgNPs@NF revealed diffraction peaks at 38.22, 44.39, 64.56, 77.49 and 81.56 that was indexed to (1 1 1), (2 0 0), (2 2 0), (3 1 1) and (2 2 2) planes of face-centered cubic (FCC) crystalline structure respectively. The particle sizes are in the range from 15.24 to 29.12 nm. The SEM images indicate that the particles are spherical in shape and have particle size in the range 53-161 nm. The nanoparticles demonstrated substantial antimicrobial activity against Gram +ve and Gram –ve harmful bacteria species viz. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The current study shows that Neem Flower extract can be used as an effective reducing and capping agents for the green synthesis of silver nanoparticles (AgNPs).
Keywords: Silver nanoparticles, neem flower extract, Powder XRD, SEMEDX, Antibacterial activity
Singh A, Gaud B, Jaybhaye S. Optimization of synthesis parameters of silver nanoparticles and its antimicrobial activity☆. Optimization of synthesis parameters of silver. 2020;3:232–236. Available from: https://doi.org/10.1016/j.mset.2019.08.004
Aritonang HF, Koleangan H, Wuntu AD. Synthesis of Silver Nanoparticles Using Aqueous Extract of Medicinal Plants' ( Impatiens balsamina and Lantana camara) Fresh Leaves and Analysis of Antimicrobial Activity. Synthesis of Silver Nanoparticles. 2019;(8642303). Available from: https://doi.org/10.1155/2019/8642303
Ibrahim HM. Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. Journal of Radiation Research and Applied Sciences. 2015;8(3):265–275. Available from: https://doi.org/10.1016/j.jrras.2015.01.007
Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology. 1998;62(2):183–193. Available from: https://doi.org/10.1016/s0378-8741(98)00055-5
Cunha BA. Antibiotic Side Effects, Medical Clinics of North America. (Vol. 85, pp. 149-185) Elsevier BV. 2001.
Vermani K, Garg S. Herbal medicines for sexually transmitted diseases and AIDS. J Ethnopharmacol .. 2002;80(1):49–66. Available from: https://doi.org/10.1016/s0378-8741(02)00009-0
Kreibig U, Vollmer M. UG, RMO, MBP, HS., eds. Optical Properties of Metal Clusters. (pp. 207-234) Berlin. Springer Berlin Heidelberg. 1995.
Slawson RM, Dyke MIV, Lee H, Trevors JT. Germanium and silver resistance, accumulation, and toxicity in microorganisms. Plasmid. 1992;27(1):72–79. Available from: https://doi.org/10.1016/0147-619x(92)90008-x
Lengke MF, Fleet ME, Gordon S. Biosynthesis of silver nanoparticles by filamentous cyanobacteria from a silver(I) nitrate complex. Langmuir. 2007;23(5):2694–2699. Available from: https://doi.org/10.1021/la0613124
Naik RR, Stringer SJ, Agarwal G, Jones SE, Stone MO. Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 2002;1(3):169–172. Available from: https://doi.org/10.1038/nmat758
Roldán MV, Frattini AL, Sanctis OA, Pellegrini NS. Characterization and applications of Ag nanoparticles in waveguides. Applied Surface Science. 2007;254(1):281–285. Available from: https://doi.org/10.1016/j.apsusc.2007.07.059
Yin H, Yamamoto T, Wada Y, Yanagida S. Large-scale and size-controlled synthesis of silver nanoparticles under microwave irradiation. Materials Chemistry and Physics. 2004;83(1):66–70. Available from: https://doi.org/10.1016/j.matchemphys.2003.09.006
Zhu Z, Kai L, Wang Y. Synthesis and applications of hyperbranched polyesters—preparation and characterization of crystalline silver nanoparticles. Materials Chemistry and Physics. 2006;96(2-3):447–453. Available from: https://doi.org/10.1016/j.matchemphys.2005.07.067
Edelstein AS, RCC. Nanomaterials, Synthesis, Properties and Applications. Bristol and Philadelphia Publishers. 1998.
Maillard M, Giorgio S, Pileni MPP. Silver Nanodisks. Advanced Materials. 2002;14(15):1084–1086. Available from: https://doi.org/10.1002/1521-4095(20020805)14:15%3C1084::AID-ADMA1084%3E3.0.CO;2-L
Mock JJ, Barbic M, Smith DR, Schultz DA, Schultz SA. Shape effects in plasmon resonance of individual colloidal silver nanoparticles. The Journal of Chemical Physics. 2002;116(15):6755–6759. Available from: https://doi.org/10.1063/1.1462610
Duran N, Marcato PL, Alves OL, Souza GID, EE. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. Journal of Nanobiotechnology. 2005;3(8). Available from: https://doi.org/10.1186/1477-3155-3-8
Mie G. Contributions to the optics of turbid media, particularly of colloidal metal solutions. Ann. Phys. 1908;25(3):377–445. Available from: https://doi.org/10.1002/andp.19083300302
N Sujith Benarzee, Varun Kumar, K Hussain Reddy. Green Synthesis, Characterization and Anti-bacterial Activity of Neem Flower Extract Assisted S ilver Nanoparticles. Karnatak University Journal of Science 54(3), (2023), 58–63. https://doi.org/10.61649/kujos/v54i3.benarzee